Biotechnology And Its Applications — DPP 1

20 NEET questions from Biotechnology And Its Applications, with answers and explanations. Attempt it as a timed test to get scoring, accuracy breakdown and mistake tracking.

Attempt this test free 20 questions · 30 minutes · +80 marks
1
You have three copies of a particular DNA molecule what technique would you use to make more copies of the molecule?
  1. A Gel electrophoresis
  2. B Sequencing
  3. C PCR
  4. D Restriction fragment analysis
Show answer

Correct answer: C

2
In gel electrophoresis the DNA fragment separate according to their size through sieving effect, which is provided by :-
  1. A Agarose gel
  2. B Nylone membrane
  3. C Polyethylene glycol
  4. D Ethidium Bromide
Show answer

Correct answer: A

3
In gel electrophoresis technique the DNA fragments are forced to move through a medium towards :-
  1. A Anode
  2. B Cathode
  3. C Both (1) and (2)
  4. D None of the above
Show answer

Correct answer: A

4
If the bacterium does not have any insert, then the presence of chromogenic substrate, it gives :-
  1. A Red coloured colonies
  2. B Colourless colonies
  3. C Blue colonies
  4. D Green colonies
Show answer

Correct answer: C

5
Which one is not a basic step in genetically modifying an organism?
  1. A Identification of DNA with desirable genes
  2. B Introduction of the identified DNA into the host
  3. C Introduction of unidentified DNA into the host
  4. D Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.
Show answer

Correct answer: C

6
The most commonly used bioreactors are of:-
  1. A Simple stirring type
  2. B Sparged stirring type
  3. C Both (1) and (2)
  4. D None of the above
Show answer

Correct answer: C

7
Which enzyme is used in PCR technique ?
  1. A Thermostable DNA polymerase
  2. B Thermostable RNA polymerase
  3. C Thermostable ligase
  4. D Thermostable vector
Show answer

Correct answer: A

8
To denature the DNA template in PCR it is heated to
  1. A $70 ^ { \circ } \mathrm { C }$
  2. B $54 ^ { \circ } \mathrm { C }$
  3. C $80 ^ { \circ } \mathrm { C }$
  4. D $94 ^ { \circ } \mathrm { C }$
Show answer

Correct answer: D

9
Alternative selectable markers developed to differentiate non-recombinants from recombinants on the basis of :-
  1. A Ability of separate them according to size
  2. B Ability to produce colour in the presence of a chromogenic substrate
  3. C Ability to not produce colour
  4. D None of the above
Show answer

Correct answer: B

10
Which of the following method of vectorless gene transfer is suitable for plants?
  1. A Biolistics method
  2. B Micro injection
  3. C Liposome mediated
  4. D Electroporation
Show answer

Correct answer: A

11
DNA cannot pass through cell membrane as it is
  1. A hydrophilic
  2. B hydrophobic
  3. C lipophilic
  4. D All the above
Show answer

Correct answer: A

12
In gel electrophoresis, separated bands of DNA are cut out from the agarose gel and extracted from the gel pieces, This step is known as :-
  1. A Blotting
  2. B Elution
  3. C Cloning
  4. D Tagging
Show answer

Correct answer: B

13
Taq. polymerase is obtained from :-
  1. A Bacillus thuriengiensis
  2. B Thermus aquaticus
  3. C Salmonella typhimurium
  4. D Eischerichia coli
Show answer

Correct answer: B

14
In gel electrophoresis the DNA fragments separate according to size (smaller the fragment size, the faster it moves) this effect is called :-
  1. A Sieving effect
  2. B Movement effect
  3. C Size effect
  4. D Spooling
Show answer

Correct answer: A

15
Agrobacterium tumifaciens a pathogen transform normal plant cells into a tumor, similarly in animals the normal cells transformed into cancerous cells by:-
  1. A Retro viruses
  2. B DNA viruses
  3. C Ribo viruses
  4. D None of these
Show answer

Correct answer: A

16
Insertional inactivation results into inactivation of which enzyme?
  1. A Transacetylase
  2. B Permease
  3. C Taq polymerase
  4. D β-galactosidase
Show answer

Correct answer: D

17
To make cell competent to take up DNA, heat shock is given to cells, the temperature of shock is :-
  1. A $30 ^ { \circ } \mathrm { C }$
  2. B $42 ^ { \circ } \mathrm { C }$
  3. C $60 ^ { \circ } \mathrm { C }$
  4. D $90 ^ { \circ } \mathrm { C }$
Show answer

Correct answer: B

18
Which of the following restriction endonuclease enzyme produce blunt end in DNA :-
  1. A
  2. B
  3. C
  4. D All the above
Show answer

Correct answer: C

19
Ti plasmid is present in :-
  1. A E.coli
  2. B Agrobacterium tumefaciens
  3. C Agrobacterium orifaciens
  4. D Vibrio cholera
Show answer

Correct answer: B

20
In PCR-technology primer is a :-
  1. A Small chemically synthesized oligonucleotide that are complementary to region of DNA
  2. B Large chemically synthesized oligonucleotide that are identical to region of DNA
  3. C Small segment of RNA
  4. D None of these
Show answer

Correct answer: A

Attempt this as a timed test

Reading answers is not the same as sitting the paper. Attempt it timed and you get your score, per-question timing, and the mistakes tracked for revision.

Start free

More Biotechnology And Its Applications tests

Biotechnology And Its Applications — DPP 2 20 Q Biotechnology And Its Applications — DPP 3 20 Q
All Biotechnology And Its Applications tests →