Biotechnology And Its Applications — DPP 2

20 NEET questions from Biotechnology And Its Applications, with answers and explanations. Attempt it as a timed test to get scoring, accuracy breakdown and mistake tracking.

Attempt this test free 20 questions · 30 minutes · +80 marks
1
Downstream processing is :-
  1. A Process of separation of DNA fragments
  2. B Process of joining the vector and the host DNA
  3. C Process including separation and purification of the product
  4. D Process of transferring DNA
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Correct answer: C

2
Which of the following enzyme will get inactivated in insertional inactivation?
  1. A Transacetylase
  2. B Permease
  3. C $\beta$-galactosidase
  4. D Taq-polymerase
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Correct answer: C

3
Cry1Ab gene produces proteins which control?
  1. A Bollworms
  2. B Corn borer
  3. C Both (1) and (2)
  4. D None of the above
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Correct answer: B

4
The linking of antibiotic resistant gene in the plasmid vector become possible with the enzyme :-
  1. A Restriction endonuclease
  2. B DNA ligase
  3. C DNA polymerase
  4. D RNA polymerase
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Correct answer: B

5
Genetically identical plants are produced by :
  1. A Somatic hybridization
  2. B Meristem culture
  3. C Micro- propagation
  4. D Embryo culture
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Correct answer: C

6
Which structure involved in genetic engineering?
  1. A Plastid
  2. B Plasmid
  3. C Codon
  4. D None
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Correct answer: B

7
Biological products are produced by transgenic animals to treat which of the following?
(a) Emphysema
(b) Phenylketonuria
(c) Cancer
(d) Cystic fibrosis
(e) Alziehmer's disease
(f) Rheumatoid arthritis
  1. A a, b, d, e
  2. B a, b, c, e, f
  3. C a, b and d
  4. D All of the above
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Correct answer: C

8
Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
  1. A restriction enzyme - Production of RFLPs
  2. B DNA ligase - that cuts DNA, creating the sticky ends
  3. C DNA polymerase - used in a polymerase chain reaction to amplify section of DNA
  4. D reverse transcriptase - production of cDNA from mRNA
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Correct answer: B

9
Genetically engineered bacteria have been used in commercial production of :-
  1. A Thyroxin
  2. B Testosterone
  3. C Human insulin
  4. D Melatonium
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Correct answer: C

10
Find the incorrect match :-
Biotechnology And Its Applications – NEET-3244
  1. A & \begin{tabular} { l } Transgenic \\ model \end{tabular} & Phenylketonuria \\ \hline
  2. B & \begin{tabular} { l } Biological \\ product \end{tabular} & $\alpha$-1-antitrypsin \\ \hline
  3. C & Vaccine safety & Transgenic mice \\ \hline
  4. D & Toxicity testing & \begin{tabular} { l } Transgenic \\ animals \end{tabular} \\ \hline \end{tabular}
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Correct answer: A

11
Which of following feature is not necessary for cloning vector?
  1. A Origin of replication
  2. B High copy number
  3. C Selectable marker
  4. D Cloning sites
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Correct answer: B

12
pBR322 which is frequently used as a vector for cloning gene is:-
  1. A an original bacterial plasmid
  2. B a modified bacterial plasmid
  3. C a viral genome
  4. D a transposon
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Correct answer: B

13
The tumour indusing capacity of Agrobacterium tumaefaciens is located in extrachromosomal plasmid and called:-
  1. A Ti - plasmid
  2. B Ri - plamid
  3. C Lambda phage
  4. D Plasmid $\mathrm { P } ^ { \mathrm { BR } } 322$
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Correct answer: A

14
Statement I: Bt toxin is produced by a bacterium Bacillus thuringiensis.
Statement II : A biopesticide has been created by expressing Bt toxin gene in plants.
  1. A Both statement I and statement II are correct.
  2. B Both statement I and statement II are incorrect.
  3. C Statement I is correct but statement II is incorrect.
  4. D Statement I is incorrect but statement II is correct.
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Correct answer: A

15
Select the incorrect match:-
  1. A Transgenic mice - Polio vaccine
  2. B Rosie cow - $\alpha$ lactalbumin gene
  3. C ssDNA/RNA probe - Gene therapy
  4. D PCR - Molecular diagnosis
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Correct answer: C

16
The basic procedure involved in the synthesis of recombinant DNA molecule is depicted below. The mistake in the procedure is :-
Biotechnology And Its Applications – NEET-3078
  1. A Enzyme polymerase is not included
  2. B The mammalian DNA is shown double stranded
  3. C Only one fragment is inserted
  4. D Two different restriction enzymes are used
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Correct answer: D

17
Which type of bioreactor is usually cylindrical or with a curved base to facilitate the mixing of the contents?
  1. A Sparged tank bioreactor
  2. B Stirred tank bioreactor
  3. C Both (1) and (2)
  4. D None of the above
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Correct answer: C

18
The critical research areas of biotechnology are-
  1. A Providing the best catalyst in the form of improved organism usually a microbe or pare enzyme.
  2. B Creating optimal conditions for a catalyst of act
  3. C Downstream processing technology.
  4. D All of these.
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Correct answer: D

19
EcoR I recognises palindromic sequence:-
  1. A
  2. B 5-AAGCTT ${ } ^ { 3 }$ ' 3-TTCGAA-5
  3. C None of the above
  4. D None of the above
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Correct answer: B

20
Agrobacterium tumifaciencs, a pathogen of several dicot plants is able to deliver a piece of DNA and it is known as :-
  1. A R-DNA
  2. B S-DNA
  3. C M-DNA
  4. D T-DNA
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Correct answer: D

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