Biotechnology Principles And Processes — DPP 1

20 NEET questions from Biotechnology Principles And Processes, with answers and explanations. Attempt it as a timed test to get scoring, accuracy breakdown and mistake tracking.

Attempt this test free 20 questions · 30 minutes · +80 marks
1
Insertional inactivation is used for :-
  1. A Selection of transformed cells
  2. B Selection of non-transformed cells
  3. C Selection of recombinants
  4. D Selection of vector
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Correct answer: C

2
Genetically modified organism are also called:
  1. A Transgenic organisms
  2. B Clones
  3. C Daughter cells
  4. D Somaclones
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Correct answer: A

3
In the following figure Which DNA is not digested ?
Biotechnology Principles And Processes – NEET-3948
Which DNA is not digested ?
  1. A Lane 1
  2. B Lane 2
  3. C Lane 3
  4. D Lane 4
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Correct answer: A

4
The DNA polymerase (Taq polymerase) used in polymerase chain reaction (PCR) has been isolated from :-
  1. A Bacteria
  2. B Plant
  3. C Nematode
  4. D Fungus
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Correct answer: A

5
Which vector can clone only a small fragment of DNA?
  1. A BAC
  2. B YAC
  3. C Plasmid
  4. D Cosmid
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Correct answer: C

6
The first restriction endonuclease was
  1. A Hind-III
  2. B Hind-II
  3. C Hind-I
  4. D Hind-IV
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Correct answer: B

7
Given below are two statements:
Statement-I : DNA is a positively charged molecule move towards the negative electrode.
Statement-II : Amplification of DNA in PCR is achieved by use of a thermostable DNA polymerase.
In the light of the above statements, choose the most appropriate answer from the options given below:
  1. A Both Statement-I and Statement-II are incorrect.
  2. B Statement-I is correct but statement-II is incorrect.
  3. C Statement-I is incorrect but Statement-II is correct.
  4. D Both Statement-I and Statement-II are correct.
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Correct answer: C

8
The construction of first r-DNA emerged from the possibility of linking a gene encoding antibiotic resistance with native plasmid of :-
  1. A Salmonella typhimurium
  2. B E. coli
  3. C Yeast
  4. D Thermus aquaticus
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Correct answer: A

9
Gel electrophoresis is used for :-
  1. A Construction of recombinant DNA by joining with cloning vectors
  2. B Purification of product
  3. C Cutting of DNA into fragments
  4. D Separation of DNA fragments according to their size
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Correct answer: D

10
For alien DNA to replicate, it needs to be a part of:
  1. A chromosome without origin of replication site
  2. B mitochondrial DNA with origin of replication site
  3. C Vector with origin of replication site
  4. D cytoplasmic DNA with origin of replication site
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Correct answer: C

11
For a DNA to function as a cloning vector the most essential requirement is :-
  1. A multiple restriction sites
  2. B several selectable markers
  3. C circular nature
  4. D 'ori' sequence
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Correct answer: D

12
The core techniques that enable birth of modern biotechnology are :-
  1. A Genetic engineering
  2. B Bioprocess engineering
  3. C Breeding
  4. D Both (A) & (B)
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Correct answer: D

13
After the formation of products in the bioreactors, it undergoes through some processes, before a finished product to be ready for marketing is called :-
  1. A Elution
  2. B Upstream processing
  3. C Downstream processing
  4. D Transformation
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Correct answer: C

14
What is the source of the Ti (Tumor inducing plasmid) which is modified and used as a cloning vector to deliver the desirable genes into plant cells?
  1. A Agrobacterium tumifaciens
  2. B Thermophilus aquaticus
  3. C Pyrococcus furiosus
  4. D Aedes aegypti
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Correct answer: A

15
Exonuclease cuts DNA from -
  1. A specific position within DNA
  2. B ends of DNA
  3. C Both (A) and (B)
  4. D None of these
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Correct answer: B

16
Given below are two statements:
Statement-I : Restriction enzyme belong to a larger class of enzymes called as nucleases.
Statement-II : Each restriction endonuclease recognise a specific palindromic nucleotide sequence in the DNA.
In the light of the above statements, choose the most appropriate answer from the options given below:
  1. A Both Statement-I and II are incorrect.
  2. B Statement-I is correct but statement-II is incorrect.
  3. C Statement-I is incorrect but Statement-II is correct.
  4. D Both Statement-I and II are correct.
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Correct answer: D

17
Among the following select which is not the tool of rDNA technology :-
  1. A Restriction
  2. B Vectors enzymes
  3. C Host
  4. D Promoter
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Correct answer: D

18
Purified DNA ultimately precipitate out after the addition of chilled ethanol. This DNA that separates out can be removed by :-
  1. A Electrophoresis
  2. B Downstream processing
  3. C PCR
  4. D Spooling
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Correct answer: D

19
The method in which alien DNA is directly injected into the nucleus of an animal cell is known as :-
  1. A Biolistics
  2. B Micro-injection
  3. C Electroporation
  4. D Heat-shock treatment
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Correct answer: B

20
Which of the following is not a feature of the cloning vector :-
  1. A Origin of replication
  2. B Many restriction sites of one restriction enzyme
  3. C Selectable marker
  4. D Restriction sites of more than one restriction enzymes
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Correct answer: B

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