Biotechnology Principles And Processes — DPP 3

20 NEET questions from Biotechnology Principles And Processes, with answers and explanations. Attempt it as a timed test to get scoring, accuracy breakdown and mistake tracking.

Attempt this test free 20 questions · 30 minutes · +80 marks
1
Match column-I with column-II.
Biotechnology Principles And Processes – NEET-4063
  1. A a-(iv), b-(ii), c-(iii), d-(i)
  2. B a-(ii), b-(iv), c-(i), d-(iii)
  3. C a-(iv), b-(i), c-(ii), d-(iii)
  4. D a-(i), b-(ii), c-(iii), d-(iv)
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Correct answer: A

2
Match column-I with column-II :
Biotechnology Principles And Processes – NEET-4085
  1. A a-(i), b-(ii), c-(iii), d-(iv)
  2. B a-(iv), b-(iii), c-(ii), d-(i)
  3. C a-(i), b-(iii), c-(ii), d-(iv)
  4. D a-(iv), b-(ii), c-(iii), d-(i)
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Correct answer: B

3
A direct method of gene transfer which is suitable for plants, in this cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA is ?
  1. A Micro-injection
  2. B Biolistics or Gene gun
  3. C Disarmed pathogen
  4. D Electroporation
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Correct answer: B

4
In 1963, two restriction endonucleases were isolated in E. coli that restricted growth of bacteriophage by -
  1. A cutting DNA
  2. B adding methyl group to DNA
  3. C removing methyl group to DNA
  4. D more than one option is correct
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Correct answer: D

5
Which of the following is/are not the applications of Biotechnology.
  1. A Test-tube baby
  2. B DNA vaccine
  3. C Correcting the defective gene
  4. D Cross-breeding
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Correct answer: D

6
After the formation of products in the bioreactors, it undergoes through a series of processes before it is ready for marketing as a finished product, is called ?
  1. A Upstream processing
  2. B Dowsteram processing
  3. C Elution
  4. D Transformation
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Correct answer: B

7
Given below are two statements:
Statement-I : Agrobacterium tumefaciens a pathogen of several monocot plants.
Statement-II : Retroviruses in animals have the ability to transform cancerous cells into normal cells.
In the light of the above statements, choose the most appropriate answer from the options given below:
  1. A Both Statement-I and Statement-II are incorrect.
  2. B Statement-I is correct but statement-II is incorrect.
  3. C Statement-I is incorrect but Statement-II is correct.
  4. D Both Statement-I and Statement-II are correct.
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Correct answer: A

8
In agarose gel electrophoresis-
  1. A DNA migrates towards negative electrode
  2. B Larger molecules migrate faster than smaller molecules
  3. C Ethidium bromide can be used to visualize the DNA fragments.
  4. D Pink coloured bands of DNA can be visualized when gel exposed to UV light
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Correct answer: C

9
EFB Stands for:
  1. A English Federation of Biology
  2. B European Federation of Biology
  3. C European Federation of Biotechnology
  4. D European Federation of Biosphere
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Correct answer: C

10
Statement-I : The normal E. coli cells do not carry resistance against any of the antibiotics.
Statement-II : Selectable marker helps in identifying and eliminating non transformants and selectively permitting the growth of the transformant.
  1. A Both statement I and statement II are incorrect.
  2. B Statement I is correct but statement II is incorrect.
  3. C Statement I is incorrect but statement II is correct.
  4. D Both statement I and statement II are correct.
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Correct answer: D

11
Transformants include -
  1. A Cells which have picked vector with foreign DNA ligated to it.
  2. B Cells which have picked up vector without foreign DNA ligated to it
  3. C Cells which have not picked up vector
  4. D Both (A) & (B)
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Correct answer: D

12
Which of the following statements in incorrect?
  1. A The first restriction endonuclease isolated was Hind III.
  2. B Restriction enzymes cut at specific base sequence is known as recognition sequence.
  3. C From to 230 strains of bacteria, 900 restriction enzymes have been isolated.
  4. D Restriction enzymes belong to a larger class of enzymes called nuclease.
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Correct answer: A

13
Genes are located on long molecules of DNA intertwined with histones. On the process of DNA isolation, histones can be removed by :
  1. A DNase
  2. B Ribonuclease
  3. C Protease
  4. D Lipase
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Correct answer: C

14
Which is not true about PCR (Polymerase Chain Reaction) ?
  1. A PCR can amplify very small amount of DNA
  2. B It can be used to detect HIV in suspects
  3. C Only RNA can be used as primer
  4. D Used to detect mutation in genes
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Correct answer: C

15
Identify the correct set of statements :
(a) When different DNAs are cut by same restriction enzyme, the resultant DNA
fragments have the same kind of sticky ends.
(b) The seperated bands of DNA are cut out from the agarose gel and extracted from
the gel piece. This step is known as elution.
(c) The RNA can be removed by treatment with protease.
(d) If any protein encoding gene is expressed in a heterologous host it is called a recombinant protein.
Choose the correct answer from options given below :
  1. A a and b only
  2. B b, c and d only
  3. C a, b and d only
  4. D a and d only
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Correct answer: C

16
Biotechnology Principles And Processes – NEET-4043
  1. A
  2. B
  3. C
  4. D
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Correct answer: D

17
Which of the following statements are incorrect?
(a) Plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.
(b) The normal E. coli cells carry resistance against antibiotics.
(c) Agarose is an artificial polymer extracted from seaweeds.
(d) The smaller the DNA fragment size, the farther it moves in gel electrophoresis.
  1. A (a) and (b)
  2. B (b) and (c)
  3. C (c) and (d)
  4. D None of the above
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Correct answer: B

18
Assertion : Continuous culture system produces a larger biomass.
Reason : It maintains the cells in their physiologically most active lag phase.
  1. A Both assertion and reason are true and reason is the correct explanation of assertion.
  2. B Both assertion and reason are true and reason is not the correct explanation of assertion.
  3. C Assertion is true but reason is false.
  4. D Both assertion and reason are false.
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Correct answer: C

19
Bioreactors are used in biosynthesis for all of the following except :
  1. A To convert raw materials into specific products, enzymes etc.
  2. B To provide optimal growth conditions.
  3. C To yield products in large quantities.
  4. D For separation and purification of products.
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Correct answer: D

20
The figure below is diagrammatic representation of pBR322 which of the given options correctly identifies its certain components.
Biotechnology Principles And Processes – NEET-3996
  1. A a-tet$^{R}$, b-amp$^{R}$, c-ori, d-BamHI
  2. B a-amp$^{R}$, b-tet$^{R}$, c-ori, d-BamHI
  3. C a-tet$^{R}$, b-amp$^{R}$, c-BamHI, d-ori
  4. D a-amp$^{R}$, b-tet$^{R}$, c-PvuII, d-EcoRI
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Correct answer: A

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