Biotechnology Principles And Processes — DPP 2

20 NEET questions from Biotechnology Principles And Processes, with answers and explanations. Attempt it as a timed test to get scoring, accuracy breakdown and mistake tracking.

Attempt this test free 20 questions · 30 minutes · +80 marks
1
Identify A, B, C and D in the given figure of E.coil cloning vector pBR322 and select the correct option :-
Biotechnology Principles And Processes – NEET-3912
  1. A
  2. B
  3. C
  4. D
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Correct answer: D

2
Match column-I with column-II.
Biotechnology Principles And Processes – NEET-3997
  1. A a-(i), b-(ii), c-(iii), d-(iv), e-(v), f-(vi)
  2. B a-(vi), b-(v), c-(iv), d-(iii), e-(ii), f-(i)
  3. C a-(ii), b-(iv), c-(i), d-(vi), e-(iii), f-(v)
  4. D a-(ii), b-(iv), c-(vi), d-(i), e-(iii), f-(v)
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Correct answer: D

3
Which of the following statements is incorrect?
  1. A The separated DNA fragments can be visualised only after staining the DNA with ethidium bromide.
  2. B Bright yellow coloured bands of DNA are seen in ethidium bromide stained gel exposed to UV light.
  3. C The separated bands of DNA are cut out from agarose gel and extracted from the gel piece.
  4. D Agarose is a natural polymer extracted from sea weeds.
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Correct answer: B

4
Assertion (A) : DNA fragments can be separated by technique known as gel electrophoresis.
Reason (R) : DNA fragments separate according to their size through sieving effect provided by agarose gel.
  1. A Both (A) and (R) are correct but (R) is not the correct explanation of (A).
  2. B (A) is not correct but (R) is correct.
  3. C (A) is correct but (R) is not correct.
  4. D Both (A) and (R) are correct and (R) is the correct explanation of (A)
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Correct answer: D

5
The key tools for recombinant DNA technology are :
  1. A Restriction enzyme, polymerase, isomerase, vectors.
  2. B Recognition enzyme, polymerase, ligase, vector.
  3. C Restriction endonuclease, polymerase, ligase, vector.
  4. D Restriction enzyme, polymerase, dehydrogenase, vector.
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Correct answer: C

6
Statement-I : DNA fragments separate according to their size through sieving effect provided by agarose gel.
Statement-II : The larger the fragment size, the farther it moves.
  1. A Both statement I and statement II are correct
  2. B Both statement I and statement II are incorrect
  3. C Statement I is correct and statement II is incorrect
  4. D Statement I is incorrect and statement II is correct
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Correct answer: C

7
If any protein encoding gene is expressed in a heterologous host, it is called as
  1. A Nonhistone protein
  2. B Histone protein
  3. C Recombinant protein
  4. D Selectable marker
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Correct answer: C

8
In vitro synthesis of multiple copies of the gene of interest is done by using :
  1. A Set of primers
  2. B Taq polymerase & nucleotides
  3. C DNA template
  4. D All of the above
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Correct answer: D

9
A thermostable DNA polymerase is isolated from a ___________, ___________.
  1. A Bacterium, Thermus arciformis
  2. B Fungus, Thermus hydrothermalis
  3. C Bacterium, Thermus aquaticus
  4. D Fungus, Thermus aquaticus
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Correct answer: C

10
Identify the correct match from column I, II and III :-
Biotechnology Principles And Processes – NEET-3984
  1. A
  2. B
  3. C
  4. D
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Correct answer: A

11
Assertion (A) : Restriction enzymes are called molecular scissors.
Reason (R): They cut DNA molecules at a particular point by recognizing a specific base sequence known as ori sequence.
  1. A Both Assertion and Reason are correct but Reason is not a correct explanation of the Assertion.
  2. B Assertion is correct and Reason is not correct.
  3. C Assertion is not correct but Reason is correct.
  4. D Both Assertion and Reason are correct and the Reason is a correct explanation of the Assertion.
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Correct answer: B

12
The figure shows DNA separated out, removed by:
Biotechnology Principles And Processes – NEET-3964
  1. A spooning
  2. B spooling
  3. C spilling
  4. D peeling
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Correct answer: B

13
Match column-I with column-II.
Biotechnology Principles And Processes – NEET-4003
  1. A a-(i), b-(ii), c-(iii), d-(iv), e-(v)
  2. B a-(iii), b-(ii), c-(i), d-(iv), e-(v)
  3. C a-(iii), b-(iv), c-(i), d-(v), e-(ii)
  4. D a-(iii), b-(ii), c-(i), d-(v), e-(iv)
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Correct answer: C

14
Assertion (A) : When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends.
Reason (R) : These can be joined together (end to end) using DNA ligases.
  1. A Both Assertion and Reason are correct but Reason is not a correct explanation of the Assertion.
  2. B Assertion is correct and Reason is not correct.
  3. C Assertion is not correct but Reason is correct.
  4. D Both Assertion and Reason are correct and the Reason is a correct explanation of the Assertion.
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Correct answer: A

15
Assertion: Bacterial cells are treated with a specific concentration of a divalent cation.
Reason: It increases the efficiency with which DNA enters the bacterium through pores in its cell membrane.
  1. A Assertion is true but Reason is false.
  2. B Both assertion and reason are false.
  3. C Both assertion and reason are true and reason is the correct explanation of assertion.
  4. D Both assertion and reason are true but reason is not the correct explanation of assertion.
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Correct answer: A

16
Identify correct labelling of sequence :
Biotechnology Principles And Processes – NEET-3981
  1. A
  2. B
  3. C
  4. D
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Correct answer: C

17
Which one of the following is not required in PCR?
  1. A Oligonucleotide primers
  2. B Helicase enzyme
  3. C DNA template
  4. D Taq polymerase
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Correct answer: B

18
Assertion (A) : DNA fragments move towards anode under an electric field.
Reason (R) : DNA fragments are negatively charged molecules.
  1. A Both Assertion and Reason are correct but Reason is not a correct explanation of the Assertion.
  2. B Assertion is correct and Reason is not correct
  3. C Assertion is not correct but Reason is correct
  4. D Both Assertion and Reason are correct and the Reason is a correct explanation of the Assertion.
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Correct answer: D

19
Statement-I : After completion of the biosynthetic stage, the product is directly marketed.
Statement-II : Process like separation and purification are involved in downstream processing.
  1. A Both statement I and statement II are correct.
  2. B Both statement I and statement II are incorrect.
  3. C Statement I is correct but statement II is incorrect.
  4. D Statement I is incorrect but statement II is correct.
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Correct answer: D

20
Identify the correct set of statements :
(a) Origin of replication is responsible for initiating replication.
(b) DNA ligase acts on cut DNA molecules and joins their ends.
(c) Plasmid is autonomously replicating circular extra chromosomal DNA.
(d) More than 900 restriction enzymes that have been isolated from over 230 strains of bacteria.
Choose the correct answer from options given below :
  1. A a and c only
  2. B b, c and d only
  3. C a, c and d only
  4. D a, b, c and d all
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Correct answer: D

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